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Phospho-EIF2S1 (Ser51) Recombinant monoclonal antibody, PBS Only (87179-2-PBS)

Phospho-EIF2S1 (Ser51) Recombinant monoclonal antibody, PBS Only

Name: Phospho-EIF2S1 (Ser51) Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 87179-2-PBS-100UG

Phospho-EIF2S1 (Ser51) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 87179-2-PBS

Clone No.252275C9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

EIF2S1 (phospho-Ser51), EIF2S1 (Ser51), eIF2α (phospho S51), p-EIF2S1, p-EIF2S1 (Ser51)

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

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Filter: AllWB

WB analysis of PC-3 using 87179-2-RR (same clone as 87179-2-PBS)
Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 87179-2-RR (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87179-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 87179-2-RR (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87179-2-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 87179-2-RR (same clone as 87179-2-PBS)
Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87179-2-RR (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87179-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87179-2-RR (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87179-2-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87179-2-PBS targets Phospho-EIF2S1 (Ser51) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Calculated Molecular Weight	36 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	NM_004094
Gene Symbol	EIF2S1
Gene ID (NCBI)	1965
RRID	AB_3745394
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P05198
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51.