RB1 Polyklonaler Antikörper

RB1 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 25628-1-AP

Synonyme

OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1

---

Galerie der Validierungsdaten

• 

  WB analysis of Jurkat using 25628-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25628-1-AP (RB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25628-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25628-1-AP (RB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 25628-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25628-1-AP (RB1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 25628-1-AP

  IP Result of anti-RB1 (IP:25628-1-AP, 4ug; Detection:25628-1-AP 1:300) with Jurkat cells lysate 3400ug.

• 

  IF Staining of HepG2 using 25628-1-AP

  Immunofluorescent analysis of HepG2 cells using 25628-1-AP (RB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A431-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

25628-1-AP bindet in WB, IP, IF, ELISA RB1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RB1 fusion protein Ag22578
• Vollständiger Name: retinoblastoma 1
• Berechnetes Molekulargewicht: 928 aa, 106 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC039060
• Gene symbol: RB1
• Gene ID (NCBI): 5925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Artif Cells Nanomed Biotechnol; Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway.; Authors - Tianqi Li
human	WB	Oncol Rep; Actin-capping protein CapG is associated with prognosis, proliferation and metastasis in human glioma.; Authors - Da-Peng Yun
human	IF	Oncol Lett; 14-3-3ε is a nuclear matrix protein, and its altered expression and localization are associated with curcumin-induced apoptosis of MG-63 cells.; Authors - Kun Lu
human	WB	Cell Death Dis; MCPIP1-mediated NFIC alternative splicing inhibits proliferation of triple-negative breast cancer via cyclin D1-Rb-E2F1 axis.; Authors - Fengxia Chen
human	WB	Biomed Pharmacother; Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.; Authors - Yudi Xiong