RBPJ Polyklonaler Antikörper

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• KD/KO Validated

RBPJ Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 14613-1-AP

Synonyme

CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, NY-REN-30, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 14613-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14613-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14613-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14613-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14613-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 14613-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14613-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14613-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14613-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14613-1-AP

  IP Result of anti-RBPJ (IP:14613-1-AP, 3ug; Detection:14613-1-AP 1:1000) with HEK-293 cells lysate 4500ug.

• 

  IHC staining of human pancreas cancer using 14613-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14613-1-AP (RBPJ antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 14613-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14613-1-AP (RBPJ antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Herzgewebe, humanes Lebergewebe, Maus-Pankreasgewebe, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 3 publications below

Produktinformation

14613-1-AP bindet in WB, IP, IHC, ELISA RBPJ und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RBPJ fusion protein Ag6206
• Vollständiger Name: recombination signal binding protein for immunoglobulin kappa J region
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC064976
• Gene symbol: RBPJ
• Gene ID (NCBI): 3516
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.

Publikationen

Species	Application	Title
mouse	IHC	Cell Biosci; Blockade of the Arid5a/IL-6/STAT3 axis underlies the anti-inflammatory effect of Rbpjl in acute pancreatitis.; Authors - Jiachen Lv
human	WB	J Biol Chem; RUNX3 maintains the mesenchymal phenotype after termination of the Notch signal.; Authors - Fu YangXin Y ; KD Validated
mouse	WB	Front Pharmacol; DAPT Attenuates Cadmium-Induced Toxicity in Mice by Inhibiting Inflammation and the Notch/HES-1 Signaling Axis.; Authors - Jia-Ying Yang
mouse	WB	Inflamm Res; Salidroside alleviates cadmium-induced toxicity in mice by restoring the notch/HES-1 and RIP1-driven inflammatory signaling axis.; Authors - Xing-Mei Shu
mouse	WB	Inflamm Res; Gisenoside Rg1 attenuates cadmium-induced neurotoxicity in vitro and in vivo by attenuating oxidative stress and inflammation; Authors - Teng‑Teng Ren
mouse	WB	Neurotoxicology; Edaravone attenuates cadmium-induced toxicity by inhibiting oxidative stress and inflammation in ICR mice.; Authors - Sheng-Rui Fan