RIC8B Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• RIC8B Recombinant monoclonal antibody (87524-1-RR)

• 

  WB analysis using 87524-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87524-1-RR (RIC8B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 87524-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87524-1-RR (RIC8B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity of 87524-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87524-1-RR against Human RIC8B were performed. The affinity constant is 1.42 nM.

RIC8B Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87524-1-RR

Clone No.252908A7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Synonyms

Brain synembryn, hSyn, Protein Ric-8B, RIC8, Synembryn-B

---

Tested Applications

• Positive WB detected in: HEK-293 cells, mouse cerebellum tissue, mouse brain tissue, rat brain tissue, fetal human brain tissue, pig brain tissue, rat cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87524-1-RR targets RIC8B in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11888

  Product name: Recombinant human RIC8B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 145-495 aa of BC034220

  Sequence: ELQGLPLLTQILESAFSIKWTDEYESAIDHNGPPLSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEELHSNAVNLLSNVPVSCLDVLICPLTHEETAQEATTLDELPSNKTAEKETVLKNNTMVYNGMNMEAIHVLLNFMEKRIDKGSSYREGLTPVLSLLTECSRAHRNIRKFLKDQVLPPLRDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAGLLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPNINLITGHLEEPMPNPIDEMTEEQKEYEAMKLVNMLDKLSRRADVKDLHQDG

• Full Name: resistance to inhibitors of cholinesterase 8 homolog B (C. elegans)
• Calculated Molecular Weight: 495 aa, 61 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC034220
• Gene Symbol: RIC8B
• Gene ID (NCBI): 55188
• RRID: AB_3745596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NVN3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ric8, also known as synembryn, is a guanine nucleotide exchange factor (GEF) that enhances variegated G protein signaling pathways, including Gα(q) and Gα(s) regulation of neurotransmission, Gα(i)-dependent mitotic spindle positioning during (asymmetric) cell division, and Gα(olf)-dependent odorant receptor signaling (PMID: 21467038). In mammals, two Ric8 homologues have been reported (Ric8A and Ric8B). Ric8B is able to potentiate G(s)/G(olf) signaling (PMID: 15829631; 16754875; 20133939).