RNF31 / HOIP Polyklonaler Antikörper

RNF31 / HOIP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 16289-1-AP

Synonyme

HOIP, RNF31, HOIL-1-interacting protein, HOIL 1 interacting protein, FLJ23501

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  WB analysis of HeLa using 16289-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16289-1-AP (HOIP antibody at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected cell cell using 16289-1-AP

  WB result of RNF31/HOIP antibody (16289-1-AP) by Emmanuel Laplantine (Institute Pasteur,Paris,France).

• 

  IHC staining of human breast cancer using 16289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16289-1-AP (HOIP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16289-1-AP (HOIP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Transfected cell cell
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below

Produktinformation

16289-1-AP bindet in WB, IHC, ELISA RNF31 / HOIP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF31 / HOIP fusion protein Ag9440
• Vollständiger Name: ring finger protein 31
• Berechnetes Molekulargewicht: 1072 aa, 120 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC017376
• Gene symbol: RNF31
• Gene ID (NCBI): 55072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ring finger protein 31 (RNF31), also named HOIL-1-interacting protein (HOIP), ZIBRA, is an atypical E3 ubiquitin ligase, belongs to ring finger protein (RNF) family. It was first cloned from breast cancer cell line MCF7 and was identified as a classical component in linear ubiquitin assembly complex (LUBAC) to facilitate NFκB signaling transduction. It also plays important regulatory functions in controlling important oncogenic pathways in breast cancer, such as ERα and p53. RNF31 is highly expressed in muscle, heart, and testis. In cells, RNF31 mainly localizes to the cytoplasm. (PMID:27460922)

Publikationen

Species	Application	Title
human	WB	Oncogene; AGO4 suppresses tumor growth by modulating autophagy and apoptosis via enhancing TRIM21-mediated ubiquitination of GRP78 in a p53-independent manner; Authors - Lin Wang
mouse	WB	J Immunol; HOIL1 Regulates Group 3 Innate Lymphoid Cells in the Colon and Protects against Systemic Dissemination, Colonic Ulceration, and Lethality from Citrobacter rodentium Infection; Authors - Victoria L Hartley
human	WB	Bioorg Med Chem; Biophysical and biological evaluation of optimized stapled peptide inhibitors of the linear ubiquitin chain assembly complex (LUBAC).; Authors - Francisco Aguilar-Alonso
human	WB	BMC Musculoskelet Disord; Polyglucosan body myopathy 1 may cause cognitive impairment: a case report from China.; Authors - Lin Chen
mouse	WB	Int Immunopharmacol; OTULIN orchestrates NCOA4-FTH1 complex to alleviate APAP-induced hepatocyte Ferroptosis; Authors - Liyuan Gao
human	WB	Arch Oral Biol; A novel inhibitor of nuclear factor kappa-B kinase subunit gamma mutation identified in an incontinentia pigmenti patient with syndromic tooth agenesis.; Authors - Shichen Sun