Tested Applications
| Positive WB detected in | C6 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11290-2-AP targets RPLP0 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1829 Product name: Recombinant human RPLP0 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC009867 Sequence: MPREDRATWKSNYFLKIIQLLDDYPKCFIVGADNVGSKQMQQIRMSLRGKAVVLMGKNTMMRKAIRGHLENNPALEKLLPHIRGNVGFVFTKEDLTEIRDMLLANKVPAAARAGAIAPCEVTVPAQNTGLGPEKTSFFQALGITTKISRGTIEILSDVQLIKTGDKVGASEATLLNMLNISPFSFGLVIQQVFDNGSIYNPEVLDITEETLHSRFLEGVRNVASVCLQIGYPTVASVPHSIINGYKRVLALSVETDYTFPLAEKVKAFLADPSAFVAAAPVAAATTAAPAAAAAPAKVEAKEESEESDEDMGFGLFD Predict reactive species |
| Full Name | ribosomal protein, large, P0 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC009867 |
| Gene Symbol | RPLP0 |
| Gene ID (NCBI) | 6175 |
| RRID | AB_2254064 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05388 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPLP0 is first identified as a cytosolic protein which takes a part in the ribosomal stalk and interacts with RPLP1 and RPLP2. It also has potential roles in molecular transport from outside into the cells. It generally presents in multiple copies on the ribosome and have isoelectric points in the range of pH 3 to 5, in contrast to most ribosomal proteins, which are single copy and basic
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPLP0 antibody 11290-2-AP | Download protocol |
| IHC protocol for RPLP0 antibody 11290-2-AP | Download protocol |
| IP protocol for RPLP0 antibody 11290-2-AP | Download protocol |
| WB protocol for RPLP0 antibody 11290-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPLP0 Polyclonal antibody (Cat# 11290-2-AP) has 33 citations across journals including Cell Metabolism, Molecular Cell, EMBO Journal, PNAS, Science Advances, Cell Reports, Genes & Development, and Frontiers in Immunology. Research fields span ribosome biology and translation, cancer (hepatocellular, breast, prostate, renal), neurodegeneration (tauopathy), metabolism, immunotherapy, cardiology, dermatology, and toxicology (alcohol, NNK). Applications are predominantly Western blot, with some RIP, IHC, IF, and ELISA.
| Species | Application | Title |
|---|---|---|
Cell Metab miR-378(∗) mediates metabolic shift in breast cancer cells via the PGC-1β/ERRγ transcriptional pathway. | ||
Mol Cell The integrated stress response regulates 18S nonfunctional rRNA decay in mammals | ||
Theranostics LRG1 inhibition promotes acute pancreatitis recovery by inducing cholecystokinin Type 1 receptor expression via Akt | ||
Cell Death Dis DNA damage-induced paraspeckle formation enhances DNA repair and tumor radioresistance by recruiting ribosomal protein P0 |
Reviews
What customers say
The product has one verified review. The reviewer used it for Western Blot on HUVEC cells (lot 00003028) and rated it 4 out of 5, describing it simply as a "good antibody." Overall, the limited feedback suggests satisfactory performance for WB applications, though no detailed comments on specificity, sensitivity, or dilution were provided.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Trinh (Verified Customer) (06-02-2020) | good antibody
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