SAR1A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SAR1A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 15350-1-AP

Synonyme

COPII associated small GTPase, GTP binding protein SAR1a, masra2, SAR1, SAR1 homolog A (S. cerevisiae), SAR1A, Sara, SARA1

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  WB analysis of mouse liver using 15350-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 15350-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 15350-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15350-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15350-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skeletal muscle using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15350-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HeLa-Zellen, HT-1080.Zellen
• Erfolgreiche Detektion in IHC: humanes Leberzirrhosegewebe, humanes Lebergewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Produktinformation

15350-1-AP bindet in WB, IHC, IP, ELISA SAR1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SAR1A fusion protein Ag7596
• Vollständiger Name: SAR1 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 23-25 kDa
• GenBank-Zugangsnummer: BC003658
• Gene symbol: SAR1A
• Gene ID (NCBI): 56681
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	J Clin Invest; Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.; Authors - Zhixiong Liu
human,mouse	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
human	WB	Proc Natl Acad Sci U S A; Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.; Authors - Liling Niu
rat	WB	J Biol Chem; The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.; Authors - Lee G D Fryer
human,mouse	IHC,WB	Clin Epigenetics; Epigenetic silencing of TMEM176A activates ERK signaling in human hepatocellular carcinoma.; Authors - Hongxia Li ; KD Validated
human	WB,IP	Biochem Biophys Res Commun; Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation.; Authors - Yuri Urai