SERCA2 Polyklonaler Antikörper

SERCA2 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 13985-1-AP

Synonyme

ATP2A2, ATP2B, Calcium pump 2, SERCA2, SR Ca(2+) ATPase 2

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  WB analysis of mouse heart using 13985-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13985-1-AP (SERCA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

13985-1-AP bindet in WB, ELISA SERCA2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERCA2 fusion protein Ag5052
• Vollständiger Name: ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
• Berechnetes Molekulargewicht: 1042 aa, 115 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC035588
• Gene symbol: SERCA2
• Gene ID (NCBI): 488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. SERCA2a and SERCA2c are mainly expressed in cardiac muscle, while SERCA2b is ubiquitously expressed in all cell types. This antibody can recognize all the above isoforms.

Publikationen

Species	Application	Title
human	WB	Circ Res; SPEG Controls Calcium Re-Uptake into the Sarcoplasmic Reticulum Through Regulating SERCA2a by Its Second Kinase-Domain.; Authors - Chao Quan
rat	WB	Mol Nutr Food Res; Sulforaphane Balances Ca2+ Homeostasis Injured by Excessive Fat via Mitochondria-Associated Membrane (MAM).; Authors - Sicong Tian
mouse	WB	Toxicol Lett; Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo.; Authors - Zhao Xiaoming X
mouse,rat	WB	J Mol Histol; Halofuginone ameliorates the susceptibility to atrial fibrillation by activating the PI3K/Akt signaling pathway; Authors - Feng Xu
mouse	WB	Life Metab; Impaired SERCA2a phosphorylation causes diabetic cardiomyopathy through impinging on cardiac contractility and precursor protein processing; Authors - Chao Quan