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SERCA2 Polyklonaler Antikörper

SERCA2 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13985-1-AP

Synonyme

ATP2A2, ATP2B, Calcium pump 2, SERCA2, SR Ca(2+) ATPase 2

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of mouse heart tissue using SERCA2 Polyclonal antibody (13985-1-AP)

    WB analysis of mouse heart using 13985-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13985-1-AP (SERCA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBMausherzgewebe

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 3 publications below

Produktinformation

13985-1-AP bindet in WB,ELISA SERCA2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen SERCA2 fusion protein Ag5052
Vollständiger Name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Berechnetes Molekulargewicht 1042 aa, 115 kDa
Beobachtetes Molekulargewicht 100 kDa
GenBank-ZugangsnummerBC035588
Gene symbol ATP2A2
Gene ID (NCBI) 488
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. SERCA2a and SERCA2c are mainly expressed in cardiac muscle, while SERCA2b is ubiquitously expressed in all cell types. This antibody can recognize all the above isoforms.

Publikationen

SpeciesApplicationTitle
humanWB

Circ Res

SPEG Controls Calcium Re-Uptake into the Sarcoplasmic Reticulum Through Regulating SERCA2a by Its Second Kinase-Domain.

Authors - Chao Quan
View Article
ratWB

Mol Nutr Food Res

Sulforaphane Balances Ca2+ Homeostasis Injured by Excessive Fat via Mitochondria-Associated Membrane (MAM).

Authors - Sicong Tian
View Article
mouseWB

Toxicol Lett

Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo.

Authors - Zhao Xiaoming X
View Article