SERCA2,ATP2A2 Monoklonaler Antikörper

SERCA2,ATP2A2 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

CloneNo.

1F11A11

Kat-Nr. : 67248-1-Ig

Synonyme

ATP2A2, SERCA2, SERCA2b, Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform, Calcium pump 2



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HepG2-Zellen, MCF-7-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IHChumanes Urothelkarzinomgewebe, Maus-Skelettmuskelgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:3000-1:20000
Immunhistochemie (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

67248-1-Ig bindet in WB, IHC, IF, ELISA SERCA2,ATP2A2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen SERCA2,ATP2A2 fusion protein Ag5052
Vollständiger Name ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Berechnetes Molekulargewicht 1042 aa, 115 kDa
Beobachtetes Molekulargewicht 120 kDa
GenBank-ZugangsnummerBC035588
Gene symbol SERCA2
Gene ID (NCBI) 488
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ATP2A2, also known as SERCA2 (Sarco/endoplasmic reticulum-type calcium transport ATPase 2), is a 110 kDa magnesium-dependent enzyme catalyzing the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Human SERCA2 gene gives rise to several alternatively spliced isoforms: SERCA2a, SERCA2b and SERCA2c. ATP2A2 is mainly expressed in the sarcoplasmic reticulum of muscle cells and the endoplasmic reticulum of most cell types.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SERCA2,ATP2A2 antibody 67248-1-IgProtokoll herunterladen
IHC protocol for SERCA2,ATP2A2 antibody 67248-1-IgProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

J Cell Physiol

microRNA-151-3p regulates slow muscle gene expression by targeting ATP2a2 in skeletal muscle cells.

Authors - Huan Wei
humanWB

Biomolecules

Urolithin A Protects Hepatocytes from Palmitic Acid-Induced ER Stress by Regulating Calcium Homeostasis in the MAM

Authors - Gayoung Ryu
mouseWB

Cell Calcium

Mannan-binding lectin deficiency augments hepatic endoplasmic reticulum stress through IP3R-controlled calcium release.

Authors - Yu Chen
ratWB

Ecotoxicol Environ Saf

Fat mass and obesity-associated gene (FTO) hypermethylation induced by decabromodiphenyl ethane causing cardiac dysfunction via glucolipid metabolism disorder.

Authors - Leqiang Gao
mouseWB

Am J Physiol Gastrointest Liver Physiol

EMC3 is critical for CFTR function and calcium mobilization in the mouse intestinal epithelium

Authors - Sarah Penrod
humanWB

J Biol Chem

Cryo-EM structure of mouse TRPML2 in lipid nanodiscs

Authors - Xiaojing Song

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Susmita (Verified Customer) (06-13-2022)

This antibody is very good for WB analysis.

  • Applications: Western Blot
  • Primary Antibody Dilution: WB: 1: 2000
  • Cell Tissue Type: PC cell line
FH

Iram (Verified Customer) (03-11-2022)

Very good antibody

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000 for western
  • Cell Tissue Type: Colon cancer cells