SLC38A1 Polyklonaler Antikörper

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• KD/KO Validated

SLC38A1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 12039-1-AP

Synonyme

Amino acid transporter A1, ATA1, NAT2, SAT1, SLC38A1, SNAT1

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  WB analysis of Jurkat cells using 12039-1-AP

  WB result of SLC38A1 antibody (12039-1-AP, 1:500) with si-Control and si-SLC38A1 transfected Jurkat cells.

• 

  WB analysis of human brain using 12039-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12039-1-AP (SLC38A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12039-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12039-1-AP (SLC38A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12039-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12039-1-AP (SLC38A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12039-1-AP

  IP result of anti-SLC38A1 (IP:12039-1-AP, 4ug; Detection:12039-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human prostate cancer using 12039-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12039-1-AP (SLC38A1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, humanes Hirngewebe, humanes Nierengewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

12039-1-AP bindet in WB, IHC, IF, IP, ELISA SLC38A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC38A1 fusion protein Ag2668
• Vollständiger Name: solute carrier family 38, member 1
• Berechnetes Molekulargewicht: 487 aa, 54 kDa
• Beobachtetes Molekulargewicht: 60-65 kDa
• GenBank-Zugangsnummer: BC010620
• Gene symbol: SLC38A1
• Gene ID (NCBI): 81539
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC38A1 (Sodium-coupled neutral amino acid transporter 1), is also named as SNAT1, which is a symporter that cotransports short-chain neutral amino acids and sodium ions from the extraccellular to the intracellular side of the cell membrane (PMID:20599747, PMID:10891391). Inhibition of SLC38A1 reduced tumor growth, cellular migration, invasion, and induced senescence in melanoma cells (PMID:35565278). SLC38A1 was identified as a positive regulator of mTORC1 in neurons, which promoted ischemic brain damage by mTOR-autophagy system (PMID:31552299). SLC38A1 is mainly expressed in brain and placenta.

Publikationen

Species	Application	Title
human	WB	Nat Commun; C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.; Authors - Dan Liu
human	IF	J Exp Med; Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine.; Authors - Yalong Wang
mouse	WB	Environ Pollut; Long-term exposure changes the environmentally relevant bis(2-ethylhexyl) phthalate to be a neuro-hazardous substance disrupting neural homeostasis in emotional and cognitive functions; Authors - Jae Soon Kang
human	WB	J Cell Biol; TFEB controls retromer expression in response to nutrient availability.; Authors - Rachel Curnock
		PLoS One; Promotion of both proliferation and neuronal differentiation in pluripotent P19 cells with stable overexpression of the glutamine transporter slc38a1.; Authors - Ogura Masato M
human	IHC	J Oncol; High Expression Levels of SLC38A1 Are Correlated with Poor Prognosis and Defective Immune Infiltration in Hepatocellular Carcinoma.; Authors - Yun Liu