SLC7A5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC7A5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 13752-1-AP

Synonyme

4F2 light chain, 4F2LC, CD98 light chain, CD98LC, D16S469E, E16, hLAT1, Integral membrane protein E16, LAT1, MPE16, SLC7A5

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Galerie der Validierungsdaten

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  WB analysis of mouse lung using 13752-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13752-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13752-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13752-1-AP (SLC7A5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13752-1-AP (SLC7A5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 13752-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13752-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, humanes Plazenta-Gewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Hodengewebe, humanes Magenkrebsgewebe, Mauslebergewebe, Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 4 publications below

Produktinformation

13752-1-AP bindet in WB, IHC, IF, ELISA SLC7A5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Kuh
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC7A5 fusion protein Ag4694
• Vollständiger Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Berechnetes Molekulargewicht: 507 aa, 55 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC039692
• Gene symbol: SLC7A5
• Gene ID (NCBI): 8140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Mol Life Sci; Regulation of branched-chain amino acid metabolism by hypoxia-inducible factor in glioblastoma.; Authors - Bo Zhang
human	IHC	J Nucl Med; PMID: 29700126; Authors - Deep Hathi
mouse	WB	Mol Metab; Resistance exercise enhances long-term mTORC1 sensitivity to leucine; Authors - Gommaar D'Hulst
human	WB	ACS Chem Neurosci; Baicalein Delays H2O2-Induced Astrocytic Senescence through Inhibition of Senescence-Associated Secretory Phenotype (SASP), Suppression of JAK2/STAT1/NF-κB Pathway, and Regulation of Leucine Metabolism.; Authors - Li Gao
human	WB,IHC	J Biol Chem; GSE1 predicts poor survival outcome in gastric cancer patients by SLC7A5 enhancement of tumor growth and metastasis.; Authors - Keshuo Ding
human	IHC	Br J Cancer; ASCT2 (SLC1A5)-dependent glutamine uptake is involved in the progression of head and neck squamous cell carcinoma.; Authors - Ze Zhang

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