SULT2B1 Polyklonaler Antikörper

SULT2B1 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12879-1-AP

Synonyme

Alcohol sulfotransferase, HSST2, ST2B1, Sulfotransferase 2B1, SULT2B1, sult2b1a, sult2b1b

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Galerie der Validierungsdaten

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  WB analysis of mouse skin using 12879-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12879-1-AP (SULT2B1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skin using 12879-1-AP

  IP Result of anti-SULT2B1 (IP:12879-1-AP, 4ug; Detection:12879-1-AP 1:400) with mouse skin tissue lysate 3200ug.

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  IHC staining of human prostate cancer using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

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  IHC staining of human prostate cancer using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.

• 

  IHC staining of human prostate cancer using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12879-1-AP (SULT2B1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50.

• 

  IHC staining of human placenta using 12879-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta slide using 12879-1-AP (SULT2B1 Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe
• Erfolgreiche IP: Maushautgewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

12879-1-AP bindet in WB, IP, IHC,ELISA SULT2B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SULT2B1 fusion protein Ag3838
• Vollständiger Name: sulfotransferase family, cytosolic, 2B, member 1
• Berechnetes Molekulargewicht: 365 aa, 41 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC034694
• Gene symbol: SULT2B1
• Gene ID (NCBI): 6820
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate. This antibody recognizes both of ULT2B1a and SULT2B1b.

Publikationen

Species	Application	Title
human	WB	J Lipid Res; Activation of liver X receptor plays a central role in antiviral actions of 25-hydroxycholesterol.; Authors - Ying Liu
mouse	WB	Drug Metab Dispos; Sulfotransferase 4A1 (SULT4A1) increases its expression in mouse neurons as they mature.; Authors - Takuyu Hashiguchi