TAOK3 Polyklonaler Antikörper

TAOK3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 10158-2-AP

Synonyme

DPK, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3

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Galerie der Validierungsdaten

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  WB analysis of human liver using 10158-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10158-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10158-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10158-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10158-2-AP

  IP Result of anti-TAOK3 (IP:10158-2-AP, 4ug; Detection:10158-2-AP 1:700) with HepG2 cells lysate 3600ug.

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  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10158-2-AP (TAOK3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10158-2-AP (TAOK3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10158-2-AP (TAOK3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 10158-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10158-2-AP (TAOK3 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, HeLa-Zellen, HepG2-Zellen, humanes Hirngewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

10158-2-AP bindet in WB, IP, IHC, ELISA TAOK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAOK3 fusion protein Ag0211
• Vollständiger Name: TAO kinase 3
• Berechnetes Molekulargewicht: 105 kDa
• Beobachtetes Molekulargewicht: 100-105 kDa
• GenBank-Zugangsnummer: BC002756
• Gene symbol: TAOK3
• Gene ID (NCBI): 51347
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAOK3(Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18 and belongs to the STE Ser/Thr protein kinase family. It acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. And this protein can be autophosphorylated(PMID:20949042).

Publikationen

Species	Application	Title
human	WB	Cell Commun Signal; Kinase shRNA screening reveals that TAOK3 enhances microtubule-targeted drug resistance of breast cancer cells via the NF-κB signaling pathway.; Authors - Tsung-Ching Lai ; KD Validated
mouse	IP	Sci Signal; The induction of SHP-1 degradation by TAOK3 ensures the responsiveness of T cells to TCR stimulation; Authors - Alexandre Poirier