TFAM Polyklonaler Antikörper

TFAM Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

40

Kat-Nr. : 19998-1-AP

Synonyme

MtTF1, mtTFA, TCF 6, TCF6, TCF6L1, TCF6L2, TCF6L3, TFAM, Transcription factor 6, Transcription factor 6 like 2

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 19998-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19998-1-AP (TFAM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19998-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19998-1-AP (TFAM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19998-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19998-1-AP (TFAM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 19998-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19998-1-AP (TFAM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 19998-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19998-1-AP (TFAM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 19998-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19998-1-AP (TFAM antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 19998-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 19998-1-AP (TFAM antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 36 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

19998-1-AP bindet in WB, IP, IHC, IF, ELISA TFAM und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: transcription factor A, mitochondrial
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: NM_003201
• Gene symbol: TFAM
• Gene ID (NCBI): 7019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TFAM, also named as TCF6L2, MtTF1, TCF6, TCF6L1, TCF6L3 and mtTFA, is a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. It is involved in mitochondrial transcription regulation. TFAM is required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. It activates transcription by binding immediately upstream of transcriptional start sites. TFAM is able to unwind and bend DNA. The molecular weighgt of TFAM isoforms are 25 kDa and 29 kDa,but the Ubiquitination of TFAM protein is about 35-37 kDa (PMID: 24607487).

Publikationen

Species	Application	Title
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
human	WB	Nat Immunol; Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.; Authors - Jing Geng
zebrafish	WB	Nucleic Acids Res; Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.; Authors - Qinghai Zhang
human	WB	Redox Biol; Defective mitochondrial ISCs biogenesis switches on IRP1 to fine tune selective mitophagy.; Authors - Hao Wu
human	WB	Proc Natl Acad Sci U S A; Mitochondrial Nuclear Retrograde Regulator 1 (MNRR1) rescues the cellular phenotype of MELAS by inducing homeostatic mechanisms.; Authors - Siddhesh Aras
mouse,human	IHC,IF	Kidney Int; Kidney epithelial targeted mitochondrial transcription factor A deficiency results in progressive mitochondrial depletion associated with severe cystic disease.; Authors - Ken Ishii ; KO Validated