TREM2 Polyklonaler Antikörper

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• KD/KO Validated

TREM2 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Rezensionen (1)

Kat-Nr. : 13483-1-AP

Synonyme

TREM 2, TREM2, Trem2a, Trem2b, Trem2c

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Galerie der Validierungsdaten

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  IHC staining of mouse brain using 13483-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13483-1-AP (TREM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13483-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13483-1-AP (TREM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 12 publications below

Produktinformation

13483-1-AP bindet in WB, IHC, IF, ELISA TREM2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TREM2 fusion protein Ag4299
• Vollständiger Name: triggering receptor expressed on myeloid cells 2
• Berechnetes Molekulargewicht: 222 aa, 25 kDa
• GenBank-Zugangsnummer: BC032362
• Gene symbol: TREM2
• Gene ID (NCBI): 54209
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213). TREM2 has a calculated molecular mass of ~25kDa, but apparent molecular mass of ~40kDa (PMID: 24078628). In addition, TREM2 has a soluble form termed as sTREM-2 with molecular weights ranging between about 24kDa and 40kDa (PMID: 18790823). Loss-of-function mutations in either TREM2 or DAP12 can cause Nasu-Hakola disease (PMID: 19302484).

Publikationen

Species	Application	Title
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
mouse	WB	Arterioscler Thromb Vasc Biol; Sexually Dimorphic Relationships Among Saa3 (Serum Amyloid A3) Inflammation, and Cholesterol Metabolism Modulate Atherosclerosis in Mice.; Authors - Alan Chait
mouse	IF	J Neuroinflammation; Edaravone ameliorates depressive and anxiety-like behaviors via Sirt1/Nrf2/HO-1/Gpx4 pathway.; Authors - Ruozhi Dang
mouse	WB,IF	Chem Biol Interact; Harpagide alleviate neuronal apoptosis and blood-brain barrier leakage by inhibiting TLR4/MyD88/NF-κB signaling pathway in Angiotensin II-induced microglial activation in vitro.; Authors - Yunwei Lu
mouse	WB,IF	Toxicol Appl Pharmacol; Methamphetamine-mediated dissemination of β-amyloid: Disturbances in endocytosis, transport and clearance of β-amyloid in microglial BV2 cells.; Authors - Liyuan Tao
mouse	IF,WB	Neural Plast; Microglia TREM2: A Potential Role in the Mechanism of Action of Electroacupuncture in an Alzheimer's Disease Animal Model.; Authors - Yujie Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hongxue (Verified Customer) (01-12-2021)

Works for IF staining

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100

TREM2 Antibody Immunofluorescence validation (1:100 dilution) in (Cat no:13483-1-AP)