TRPC1 Polyklonaler Antikörper

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• KD/KO Validated

TRPC1 Polyklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 19482-1-AP

Synonyme

HTRP 1, Transient receptor protein 1, TRP 1, TRP1, TRPC1

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Galerie der Validierungsdaten

• 

  WB analysis of PC-3 using 19482-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 19482-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19482-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 19482-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19482-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 19482-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 19482-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19482-1-AP (TRPC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 19482-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19482-1-AP (TRPC1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-12 using 19482-1-AP

  Immunofluorescent analysis of PC-12 cells using 19482-1-AP (TRPC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of PC-3 using 19482-1-AP

  1X10^6 PC-3 cells were stained with 0.2ug TRPC1 antibody (19482-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, humanes Hirngewebe, humanes Hodengewebe, Mauslebergewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-12-Zellen
• Erfolgreiche Detektion in FC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

19482-1-AP bindet in WB, IP, IHC, IF, FC, ELISA TRPC1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: transient receptor potential cation channel, subfamily C, member 1
• Berechnetes Molekulargewicht: 91 kDa
• Beobachtetes Molekulargewicht: 90 kDa, 130 kDa
• GenBank-Zugangsnummer: NM_003304
• Gene symbol: TRPC1
• Gene ID (NCBI): 7220
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TRPC1, also named as TRP1, belongs to the transient receptor family and STrpC subfamily. TRPC1 is thought to form a receptor-activated non-selective calcium permeant cation channel. It has been reported that the mGluR1 evoked slow EPSC is mediated by the TRPC1 cation channel. It is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors.

Publikationen

Species	Application	Title
human	WB	Cell Res; Direct control of store-operated calcium channels by ultrafast laser.; Authors - Pan Cheng ; KD Validated
mouse	WB,IF	J Neuroinflammation; Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.; Authors - Shiji Huo
human	WB	J Biomed Sci; Chemoresistant ovarian cancer enhances its migration abilities by increasing store-operated Ca2+ entry-mediated turnover of focal adhesions.; Authors - Ho-Kai Huang
rat	WB	Sci Rep; Homer binds to Orai1 and TRPC channels in the neointima and regulates vascular smooth muscle cell migration and proliferation.; Authors - Shuping Jia
rat	WB	J Biochem Mol Toxicol; Galectin-3 mediates pulmonary vascular endothelial cell dynamics via TRPC1/4 under acute hypoxia.; Authors - Yumei Li
mouse	WB	Int J Mol Sci; STIM1 Knockout Enhances PDGF-Mediated Ca2+ Signaling through Upregulation of the PDGFR⁻PLCγ⁻STIM2 Cascade.; Authors - Tzu-Yu Huang

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