TSG101 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TSG101 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (3)

Anwendung

WB, RIP, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

205

Rezensionen (1)

Kat-Nr. : 14497-1-AP

Synonyme

ESCRT I complex subunit TSG101, TSG10, TSG101, tumor susceptibility gene 101, VPS23

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Galerie der Validierungsdaten

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  WB analysis using 14497-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14497-1-AP (TSG101 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 14497-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14497-1-AP (TSG101 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14497-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14497-1-AP (TSG101 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14497-1-AP

  IP Result of anti-TSG101 (IP:14497-1-AP, 3ug; Detection:14497-1-AP 1:500) with HeLa cells lysate 1320ug.

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  IHC staining of human colon cancer using 14497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14497-1-AP (TSG101 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14497-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14497-1-AP (TSG101 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 14497-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14497-1-AP (TSG101 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14497-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14497-1-AP (TSG101 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14497-1-AP

  Immunofluorescent analysis of Hela cells, using TSG101 antibody 14497-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 14497-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TSG101 antibody (14497-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:10-1:100
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 191 publications below
• IHC: See 3 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• FC: See 3 publications below
• RIP: See 1 publications below

Produktinformation

14497-1-AP bindet in WB, RIP, IP, IHC, IF, FC, ELISA TSG101 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TSG101 fusion protein Ag5920
• Vollständiger Name: tumor susceptibility gene 101
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC002487
• Gene symbol: TSG101
• Gene ID (NCBI): 7251
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

Publikationen

Species	Application	Title
human, mouse	WB, IHC	Nature; Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.; Authors - Lin Zhang
human	WB	Cell; Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways.; Authors - Mirjam C Boelens
human	WB	Cell; Exosome RNA Unshielding Couples Stromal Activation to Pattern Recognition Receptor Signaling in Cancer.; Authors - Barzin Y Nabet
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier ; KD Validated
mouse,human	WB	ACS Nano; Mesenchymal Stem Cell-Derived Extracellular Vesicles Attenuate Mitochondrial Damage and Inflammation by Stabilizing Mitochondrial DNA.; Authors - Meng Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Pasquale (Verified Customer) (11-06-2019)

The antibody recognize the exosomal marker TSG101 as claimed. We tested it by Western blotting in our exosome preps isolated from murine brains and it gave the signal at the correct molecular weight in the fractions that are enriched in exosomes, so it seems to be specific. However, we report here some drawbacks that we have experienced using this antibody (see the file attached):1. There is a strong cross-reactivity of the antibody with the molecular weight marker we use (The Bio-Rad Dual Color Standard, cat. number 1610374)2. The signal over noise ratio is a little low (the background coming from the membrane is pretty high)

• Applications: Western Blot,
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Murine brain extracellular vesicles fractions

TSG101 Antibody Western Blot, validation (1 to 1000 dilution) in Murine brain extracellular vesicles fractions (Cat no:14497-1-AP)

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