Anticorps Polyclonal de lapin anti-AHR

• Phare
• Validé par KD/KO

AHR Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

45

Avis (2)

N° de cat : 17840-1-AP

Synonymes

Ah receptor, AHR, aryl hydrocarbon receptor, bHLHe76

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Galerie de données de validation

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  WB analysis using 17840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17840-1-AP (AHR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17840-1-AP

  WB result of AHR antibody (17840-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHR transfected HepG2 cells.

• 

  IP experiment of PC-3 using 17840-1-AP

  IP Result of anti-AHR (IP:17840-1-AP, 4ug; Detection:17840-1-AP 1:500) with PC-3 cells lysate 1880ug.

• 

  IHC staining of human lung cancer using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17840-1-AP (AHR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17840-1-AP (AHR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung cancer using 17840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17840-1-AP (AHR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562, cellules MCF-7
• Résultats positifs en IP: cellules PC-3
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cérébral humain, tissu d'intestin grêle de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 41 publications below
• IHC: See 10 publications below
• IF: See 9 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

17840-1-AP cible AHR dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AHR Protéine recombinante Ag12193
• Nom complet: aryl hydrocarbon receptor
• Masse moléculaire calculée: 848 aa, 96 kDa
• Poids moléculaire observé: 105-110 kDa
• Numéro d’acquisition GenBank: BC070080
• Symbole du gène: AHR
• Identification du gène (NCBI): 196
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism [PMID:18483242]. It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH)17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling [PMID:20423157,18540824]. It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)

Publications

Species	Application	Title
mouse	IF	J Clin Invest; Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy; Authors - Frank L. van de Veerdonk
human	WB	J Immunother Cancer; GCH1 induces immunosuppression through metabolic reprogramming and IDO1 upregulation in triple-negative breast cancer.; Authors - Jin-Li Wei ; KD Validated
human,mouse	WB	Theranostics; Activating AhR alleviates cognitive deficits of Alzheimer's disease model mice by upregulating endogenous Aβ catabolic enzyme Neprilysin.; Authors - Cheng Qian ; KD Validated
mouse	WB	Br J Pharmacol; AhR-STAT3-Hmox1/Ptgs2 signaling pathway may restrict ferroptosis and improve hMSC accumulation and efficacy in mice liver; Authors - Li Han
mouse	WB	mSystems; Impaired Intestinal Akkermansia muciniphila and Aryl Hydrocarbon Receptor Ligands Contribute to Nonalcoholic Fatty Liver Disease in Mice.; Authors - Zunji Shi
mouse	WB,IHC,IF	J Invest Dermatol; Aryl Hydrocarbon Receptor in Cutaneous Vascular Endothelial Cells Restricts Psoriasis Development by Negatively Regulating Neutrophil Recruitment.; Authors - Zhenlai Zhu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Hala (Verified Customer) (04-13-2021)

works good!

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepG2, U937

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