Anticorps Monoclonal anti-AKT1/3

• Phare
• Validé par KD/KO

AKT1/3 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

51

CloneNo.

3G5E11

N° de cat : 60203-1-Ig

Synonymes

AKT, AKT 1, AKT1, AKT1/3, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha

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Galerie de données de validation

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  WB analysis of MCF-7 using 60203-1-Ig

  WB result of AKT1/3 antibody (60203-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AKT1/3 transfected MCF-7 cells.

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  WB analysis of HeLa using 60203-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60203-1-Ig (AKT1/3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 60203-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 60203-1-Ig

  IP Result of anti-AKT1/3 (IP:60203-1-Ig, 5ug; Detection:60203-1-Ig 1:600) with mouse brain tissue lysate 3440ug.

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  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 10x lens).

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  IHC staining of human breast cancer using 60203-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60203-1-Ig (AKT1/3 antibody at dilution of 1:500 (under 40x lens).

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  IF Staining of MCF-7 using 60203-1-Ig

  Immunofluorescent analysis of MCF-7 cells, using AKT1 antibody 60203-1-Ig at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of MCF-7 using 60203-1-Ig

  1X10^6 MCF-7 cells were stained with 0.2ug AKT1/3 antibody (60203-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu hépatique de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 50 publications below
• IF: See 1 publications below

Informations sur le produit

60203-1-Ig cible AKT1/3 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: AKT1/3 Protéine recombinante Ag16695
• Nom complet: v-akt murine thymoma viral oncogene homolog 1
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56-62 kDa
• Numéro d’acquisition GenBank: BC000479
• Symbole du gène: AKT1
• Identification du gène (NCBI): 207
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The serine-threonine protein kinase AKT is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT, which then phosphorylates and inactivates components of the apoptotic machinery.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Exposure to Static Magnetic and Electric Fields Treats Type 2 Diabetes.; Authors - Calvin S Carter
human	WB	J Exp Clin Cancer Res; Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization; Authors - Yuanshan Yao
human	WB	Sci Total Environ; Effects of triclosan exposure on stem cells from human exfoliated deciduous teeth (SHED) fate; Authors - Shiwen Deng
mouse	WB	Autophagy; BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.; Authors - Simone C da Silva Rosa
human	WB	Cancer Lett; Endogenous production of C-C motif chemokine ligand 2 by nasopharyngeal carcinoma cells drives radioresistance-associated metastasis.; Authors - Shan-Shan Guo
huamn,mouse	WB	Cell Death Dis; Elaiophylin reduces body weight and lowers glucose levels in obese mice by activating AMPK; Authors - Ruoxuan Bao