Anticorps Polyclonal de lapin anti-Angiopoietin 1

• Phare
• Validé par KD/KO

Angiopoietin 1 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

27

N° de cat : 23302-1-AP

Synonymes

AGP1, AGPT, ANG 1, ANG1, angiopoietin 1, Angiopoietin-1, ANGPT1, KIAA0003

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Galerie de données de validation

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  WB analysis using 23302-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 23302-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 23302-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 23302-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23302-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23302-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 23302-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23302-1-AP (Angiopoietin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HUVEC, cellules A375, cellules C6, cellules HeLa, cellules HepG2, cellules K-562, cellules MG U-87, tissu cardiaque de rat, tissu cardiaque de souris, tissu hépatique de souris
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 25 publications below
• IF: See 2 publications below

Informations sur le produit

23302-1-AP cible Angiopoietin 1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Angiopoietin 1 Protéine recombinante Ag18829
• Nom complet: angiopoietin 1
• Masse moléculaire calculée: 498 aa, 58 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC152411
• Symbole du gène: ANGPT1
• Identification du gène (NCBI): 284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Angiopoietin 1 is a 70-kDa secreted glycoprotein generated from vascular mural cells, pericytes, and certain other cells. Angiopoietin 1 participates in vascular differentiation through angiogenesis, which is the process of the growth and remodelling of existing vessels. Angiopoietin 1 is also involved in the maintenance and turnover of blood vessels in mature animals. Angiopoietin 1 binds to and activates the TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation, playing an important role in the regulation of angiogenesis. Overexpression of Angiopoietin 1 has been proven to occur in malignant glioblastoma, neuroblastoma, non-small cell lung cancer, and other tumors.

Publications

Species	Application	Title
human	WB	Biomater Res; High yield engineered nanovesicles from ADSC with enriched miR-21-5p promote angiogenesis in adipose tissue regeneration; Authors - Di Sun
human	WB,IF	Mol Ther Nucleic Acids; Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.; Authors - Ying Wang
human,mouse	WB	Neural Regen Res; Endorepellin downregulation promotes angiogenesis after experimental traumatic brain injury; Authors - Qian Zhang
human	WB	J Cell Mol Med; IGFBP-4 enhances VEGF-induced angiogenesis in a mouse model of myocardial infarction.; Authors - Da Wo
mouse	WB	Br J Pharmacol; Canonical hedgehog signaling regulates hepatic stellate cell-mediated angiogenesis in liver fibrosis.; Authors - Feng Zhang
rat	WB	Cell Physiol Biochem; Neuregulin-1 Promotes Myocardial Angiogenesis in the Rat Model of Diabetic Cardiomyopathy.; Authors - Chun Gui