Anticorps Polyclonal de lapin anti-ATG9A

ATG9A Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (2)

N° de cat : 26276-1-AP

Synonymes

APG9 like 1, APG9L1, ATG9A, Autophagy related protein 9A, MGD3208

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Galerie de données de validation

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  WB analysis using 26276-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26276-1-AP (ATG9A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 26276-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26276-1-AP (ATG9A Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 26276-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 26276-1-AP (ATG9A Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 26276-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26276-1-AP (ATG9A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human brain using 26276-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26276-1-AP (ATG9A Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of mouse brain using 26276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 26276-1-AP (ATG9A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 26276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 26276-1-AP (ATG9A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cardiaque humain, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

26276-1-AP cible ATG9A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ATG9A Protéine recombinante Ag24212
• Nom complet: ATG9 autophagy related 9 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 837 aa, 94 kDa
• Poids moléculaire observé: 94 kDa
• Numéro d’acquisition GenBank: BC065534
• Symbole du gène: ATG9A
• Identification du gène (NCBI): 79065
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ATG9A is the only transmembrane ATG protein essential for autophagy. It plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS). It has been reported that ATG9A expression is increased in oral squamous cell carcinoma and breast cancers. The inhibition of ATG9A can lead to an inhibition of cancer cell proliferation and invasion.(PMID: 29437695, 29568063)

Publications

Species	Application	Title
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
rat	WB	Acta Pharm Sin B; MicroRNA-34c-5p provokes isoprenaline-induced cardiac hypertrophy by modulating autophagy via targeting ATG4B.; Authors - Yuhong Zhang
mouse	WB	Front Immunol; MicroRNA-34a Inhibition Alleviates Lung Injury in Cecal Ligation and Puncture Induced Septic Mice.; Authors - Song Chen
human	WB	DNA Cell Biol; Long Noncoding RNA KIF9-AS1 Regulates Transforming Growth Factor-β and Autophagy Signaling to Enhance Renal Cell Carcinoma Chemoresistance via microRNA-497-5p.; Authors - Yichen Jin
mouse	WB	Mol Neurobiol; RAB7A GTPase Is Involved in Mitophagosome Formation and Autophagosome-Lysosome Fusion in N2a Cells Treated with the Prion Protein Fragment 106-126; Authors - Zhiping Li
		Cell Biol Int; TFAM knockdown undermines SQSTM1 mRNA stability but retards autophagy flux and inhibits tumor cells proliferation under starvation conditions; Authors - Xu Jiang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon