Anticorps Monoclonal anti-Bcl2

Bcl2 Monoclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

souris et plus (2)

Applications

WB, IP, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

18

CloneNo.

1B5B9

N° de cat : 66799-1-Ig

Synonymes

AW986256, B cell leukemia/lymphoma 2, Bcl2, C430015F12Rik, D630044D05Rik, D830018M01Rik

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Galerie de données de validation

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  WB analysis using 66799-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66799-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 66799-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66799-1-Ig (Bcl2 antibody) at dilution of 1:1400 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 66799-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Bcl2 antibody (66799-1-Ig, Clone: 1B5B9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of NIH/3T3 using 66799-1-Ig

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse Bcl2 (66799-1-Ig, Clone:1B5B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules 4T1, cellules C2C12, cellules RAW 264.7, tissu cérébral de souris
• Résultats positifs en IF: cellules NIH/3T3,
• Résultats positifs en cytométrie: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 17 publications below
• IP: See 1 publications below

Informations sur le produit

66799-1-Ig cible Bcl2 dans les applications de WB, IP, IF, FC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Bcl2 Protéine recombinante Ag24261
• Nom complet: B-cell leukemia/lymphoma 2
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: NM-009741
• Symbole du gène: Bcl2
• Identification du gène (NCBI): 12043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Bcl2 is a member of the B cell lymphoma 2 protein family. Members of this family regulate cell death in multiple cell types and can have either proapoptotic or antiapoptotic activities. The protein encoded by this gene inhibits mitochondrial-mediated apoptosis. This protein is an integral outer mitochondrial membrane protein that functions as part of signaling pathway that controls mitochondrial permeability in response to apoptotic stimuli. This protein may also play a role in neuron cell survival and autophagy. Abnormal expression and chromosomal translocations of this gene are associated with cancer progression in numerous tissues.

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Puerarin prevents sepsis-associated encephalopathy by regulating the AKT1 pathway in microglia; Authors - Shao-Peng Lin
human,mouse	WB	Biomed Pharmacother; Lychee seed polyphenol ameliorates DR via inhibiting inflammasome/apoptosis and angiogenesis in hRECs and db/db mice; Authors - Xiao-Hong Xiang
		Redox Biol; Resveratrol as a new inhibitor of immunoproteasome prevents PTEN degradation and attenuates cardiac hypertrophy after pressure overload.; Authors - Chen Chen
mouse	WB	Antioxidants (Basel); Continuous Monochromatic Blue Light Exacerbates High-Fat Diet-Induced Kidney Injury via Corticosterone-Mediated Oxidative Stress; Authors - Wenji Ren
mouse	WB, IP	Cell Prolif; Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy.; Authors - Li Chen
mouse	WB	Int J Mol Sci; Elsholtzia bodinieri Vaniot Ameliorated Acute Lung Injury by NQO1, BCL2 and PTGS2 In Silico and In Vitro Analyses; Authors - Jin Sun