Anticorps Monoclonal anti-CA9

CA9 Monoclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

CloneNo.

1G4G5

Avis (1)

N° de cat : 66243-1-Ig

Synonymes

CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250

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Galerie de données de validation

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  WB analysis of U-87 MG using 66243-1-Ig

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 66243-1-Ig (CA9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig stomach using 66243-1-Ig

  pig stomach tissue were subjected to SDS PAGE followed by western blot with 66243-1-Ig (CA9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66243-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66243-1-Ig (CA9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 66243-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66243-1-Ig (CA9 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 66243-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 66243-1-Ig (CA9 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HEK-293 using 66243-1-Ig

  1X10^6 HEK-293 cells were stained with 0.20 ug/test Anti-Human CA9 (66243-1-Ig, Clone:1G4G5) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. Fixed with90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules MG U-87, tissu cérébral de porc, tissu d'estomac de porc
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

66243-1-Ig cible CA9 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, souris

• Réactivité: Humain, Lapin, porc, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CA9 Protéine recombinante Ag1540
• Nom complet: carbonic anhydrase IX
• Masse moléculaire calculée: 459 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC014950
• Symbole du gène: CA9
• Identification du gène (NCBI): 768
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CA9(Carbonic anhydrase 9) may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker for a cervical neoplasia(PMID:18703501).It is a tumor-associated antigen that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma.The protein is presentboth on the plasma membrane and in the nucleus of cells and has the molecular.It was observed the 54KDa and 58 Kda bands in A549.

Publications

Species	Application	Title
human	IF	Mol Oncol; Identification and validation of a combined hypoxia and immune index for triple-negative breast cancer.; Authors - Shaoquan Zheng
human	IF	Elife; Hypoxia-induced proteasomal degradation of DBC1 by SIAH2 in breast cancer progression; Authors - Qiangqiang Liu
human	WB	Cell Rep; ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis; Authors - Fangquan Chen
human	IHC	Int J Gen Med; Identification of Hypoxia-Associated Signature in Colon Cancer to Assess Tumor Immune Microenvironment and Predict Prognosis Based on 14 Hypoxia-Associated Genes; Authors - Peng Chen
pig	IHC	J Transl Med; Tumor microenvironment in a minipig model of spinal cord glioma; Authors - Muhibullah S Tora

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (04-16-2019)

Specific band in hypoxic mouse kidney.Note: The lower part of the blot (<25 kDa) was stained for a hypoxic marker (Ndufa4l2).

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse kidney, hypoxic (lane1), normoxic (lane2)

CA9 Antibody Western Blot, validation (1:1000 dilution) in Mouse kidney, hypoxic (lane1), normoxic (lane2) (Cat no:66243-1-Ig)