Anticorps Monoclonal anti-Endoglin/CD105

Endoglin/CD105 Monoclonal Antibody for IF-P, FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

IF-P, FC

Conjugaison

Non conjugué

Publications(1)

CloneNo.

MJ7/18

N° de cat : 65048-1-Ig

Synonymes

CD105, Edg, endoglin, Eng, MJ7/18

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Galerie de données de validation

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  IF Staining of mouse liver using 65048-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CD105 antibody (65048-1-Ig, Clone: MJ7/18 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of bEnd.3 using 65048-1-Ig

  1X10^6 bEnd.3 cells were surface stained with 0.25 ug Anti-Mouse CD105 (65048-1-Ig, Clone:MJ7/18) and FITC anti-rat IgG2a Antibody at dilution 1:100 (red), or 0.25 ug Control Antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en IF-P: tissu hépatique de souris,
• Résultats positifs en cytométrie: cellules bEnd.3,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

65048-1-Ig cible Endoglin/CD105 dans les applications de IF-P, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peau de souris enflammée
• Nom complet: endoglin
• Numéro d’acquisition GenBank: BC029080
• Symbole du gène: CD105
• Identification du gène (NCBI): 13805
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: Borate buffered saline
• Conditions de stockage: Store at 2-8°C. Stable for one year after shipment.

Informations générales

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Publications

Species	Application	Title
mouse	IF	Bone; Radial extracorporeal shockwave promotes osteogenesis-angiogenesis coupling of bone marrow stromal cells from senile osteoporosis via activating the Piezo1/CaMKII/CREB axis; Authors - Bo Wang

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