Anticorps Monoclonal anti-CD11b
CD11b Monoclonal Antibody for FC
Hôte / Isotype
Mouse / IgG1, kappa
Réactivité testée
Humain
Applications
FC
Conjugaison
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
ICRF44
N° de cat : CL750-65116
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en cytométrie | CMSP humaines, |
Dilution recommandée
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
CL750-65116 cible CD11b dans les applications de FC et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Hôte / Isotype | Mouse / IgG1, kappa |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | Cellules synoviales rhumatoïdes et monocytes humains |
| Nom complet | integrin, alpha M (complement component 3 receptor 3 subunit) |
| Masse moléculaire calculée | 1152 aa, 127 kDa |
| Numéro d’acquisition GenBank | BC096346 |
| Symbole du gène | CD11b |
| Identification du gène (NCBI) | 3684 |
| Conjugaison | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Forme | Liquide |
| Méthode de purification | Purification par affinité |
| Tampon de stockage | PBS with 0.09% sodium azide and 0.5% BSA |
| Conditions de stockage | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Informations générales
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocole
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 750 CD11b antibody CL750-65116 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Cyril (Verified Customer) (11-19-2024) | It is complicated to conclude on the use of this antibody. Indeed, the laser of our Attune NxT does not allow to detect the Coralyte 750 (maximum 17% emission). We will soon test another format for the project.
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