Anticorps Monoclonal anti-CD11b

CD11b Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

Humain, souris

Applications

FC

Conjugaison

PerCP-Cyanine5.5 Fluorescent Dye

CloneNo.

M1/70

N° de cat : CPY5-65055

Synonymes

CR3, CR3A, integrin alpha M, Itgam, Ly 40

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Galerie de données de validation

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  FC experiment of mouse bone marrow cells using CPY5-65055

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug PerCP-Cyanine5.5 Anti-Mouse CD11b (M1/70) (CPY5-65055, Clone:M1/70) or unstained. Cells were then stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (CL647-65140, Clone: RB6-8C5). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CPY5-65055 cible CD11b dans les applications de FC et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes T spléniques de souris C57BL/10 et splénocytes C57BL/10 activés par la concanavaline A
• Nom complet: integrin alpha M
• Masse moléculaire calculée: 127 kDa
• Numéro d’acquisition GenBank: BC156991
• Symbole du gène: CD11b
• Identification du gène (NCBI): 16409
• Conjugaison: PerCP-Cyanine5.5 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 482 nm / 690 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

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