Anticorps Monoclonal anti-CD11b

CD11b Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

Humain, souris

Applications

IF, FC

Conjugaison

PE Fluorescent Dye

Publications(4)

CloneNo.

M1/70

N° de cat : PE-65055

Synonymes

CD11b, CD11b/CD18, CR3, CR3A, F730045J24Rik, integrin alpha M, Itgam, Ly 40, Mac 1, Mac 1a, MAC1

---

Galerie de données de validation

• 

  FC experiment of mouse bone marrow cells using PE-65055

  1x10^6 mouse bone marrow cells were surface stained with 0.2 ug PE Anti-Mouse CD11b (M1/70) (PE-65055, Clone:M1/70) or 0.2 ug PE Rat IgG2b Isotype Control (LTF-2) (PE-65211, Clone: LTF-2), and 0.2 ug CoraLite® Plus 647 Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) (CL647-65140, Clone: RB6-8C5). Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: cellules médullaires de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

PE-65055 cible CD11b dans les applications de IF, FC et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes T spléniques de souris C57BL/10 et splénocytes C57BL/10 activés par la concanavaline A
• Nom complet: integrin alpha M
• Masse moléculaire calculée: 127 kDa
• Numéro d’acquisition GenBank: BC156991
• Symbole du gène: CD11b
• Identification du gène (NCBI): 16409
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).

Publications

Species	Application	Title
mouse	FC	Biomed Pharmacother; Resveratrol alleviated neuroinflammation induced by pseudorabies virus infection through regulating microglial M1/M2 polarization; Authors - Xiangxiu Chen
mouse	IF	Bioact Mater; Reprogramming exosomes for immunity-remodeled photodynamic therapy against non-small cell lung cancer; Authors - Jiao Guo
mouse	IF	Phytomedicine; Xibining inhibition of the PI3K-AKT pathway reduces M1 macrophage polarization to ameliorate KOA synovial inflammation and nociceptive sensitization; Authors - Lishi Jie
mouse	IF	Pharmaceutics; Neutrophil-Camouflaged Stealth Liposomes for Photothermal-Induced Tumor Immunotherapy Through Intratumoral Bacterial Activation; Authors - Xinxin Chen

{{ptg:RelatedPrimaryAntibodies}}