Anticorps Monoclonal anti-CD11c

CD11c Monoclonal Antibody for FC, IF

Hôte / Isotype

Armenian Hamster / IgG

Réactivité testée

souris

Applications

IF, FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

N418

N° de cat : CL488-65130

Synonymes

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418

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Galerie de données de validation

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  IF Staining of mouse splenocytes using CL488-65130

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488 CD11c antibody (CL488-65130, Clone: N418 ) at dilution of 1:400.

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  FC experiment of mouse splenocytes using CL488-65130

  1X10^6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11c (CL488-65130, Clone: N418) or 0.25 ug isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en IF: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-65130 cible CD11c dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Armenian Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cellules dendritiques de rate de souris
• Nom complet: integrin alpha X
• Numéro d’acquisition GenBank: BC167225
• Symbole du gène: Itgax
• Identification du gène (NCBI): 16411
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).