Anticorps Monoclonal anti-CD16/CD32

CD16/CD32 Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Rat / IgG2a, lambda

Réactivité testée

souris

Applications

IF/ICC, FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

93

N° de cat : CL647-65057

Synonymes

CD16/32, CD32, 93, Fc gamma receptor IIB, Fc-gamma RII

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Galerie de données de validation

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  IF Staining of mouse splenocytes using CL647-65057

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647 CD16/32 antibody (CL647-65057, Clone: 93 ) at dilution of 1:100.

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  FC experiment of mouse splenocytes using CL647-65057

  1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD16/32 (CL647-65057, Clone:93), or 0.5 ug Control Antibody. Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65057 cible CD16/CD32 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, lambda
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lymphocytes B VH81X Tg et lignée de cellules stromales T220
• Nom complet: Fc receptor, IgG, low affinity IIb
• Numéro d’acquisition GenBank: BC038070
• Symbole du gène: CD16/32
• Identification du gène (NCBI): 14130
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD16 (FcγRIII) is a 50-70 kDa low affinity Fc receptor found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16 mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. CD32 (FcγRII) is a 40 kD transmembrane glycoprotein that binds to the Fc region of IgG with low affinity. CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.

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