Anticorps Monoclonal anti-CD25/IL-2RA

CD25/IL-2RA Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

APC Fluorescent Dye

CloneNo.

BC96

N° de cat : APC-65096

Synonymes

CD25, BC96, IL 2 RA, IL 2 receptor subunit alpha, IL 2R subunit alpha

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Galerie de données de validation

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  FC experiment of human PBMCs using APC-65096

  1x10^6 untreated (left) or PHA-treated (3 days) (right) human PBMCs were surface stained with 5 ul APC Anti-Human CD25/IL-2RA (BC96) (APC-65096, Clone: BC96) and CoraLite® Plus 488 Anti-Human CD4. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

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  FC experiment of human PBMCs using APC-65096

  1x10^6 PHA-treated (3 days) human PBMCs were surface stained with 5 ul APC Anti-Human CD25/IL-2RA (BC96) (APC-65096, Clone: BC96) (red) or APC Mouse IgG1 Isotype Control (MOPC-21) (APC-65124, Clone: MOPC-21)(blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul APC Anti-Human CD25/IL-2RA (BC96) (APC-65096, Clone: BC96) (black, dashed). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

APC-65096 cible CD25/IL-2RA dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: interleukin 2 receptor, alpha
• Masse moléculaire calculée: 272 aa, 31 kDa
• Numéro d’acquisition GenBank: BC156627
• Symbole du gène: IL2RA
• Identification du gène (NCBI): 3559
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.1% gelatin
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells and a subset of human CD4+ memory T cells (PMID: 22585674).

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