Anticorps Monoclonal anti-CD27

CD27 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

O323

N° de cat : CL647-65132

Synonymes

CD27, CD27 antigen, CD27 molecule, CD27L receptor, S152, T cell activation antigen CD27, T14, TNFRSF7, Tp55



Applications testées

Résultats positifs en cytométrielymphocytes de sang périphérique humain,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65132 cible CD27 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène s/o
Nom complet CD27 molecule
Masse moléculaire calculée 29 kDa
Numéro d’acquisition GenBankBC012160
Symbole du gène CD27
Identification du gène (NCBI) 939
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

Protocole

Product Specific Protocols
FC protocol for CL Plus 647 CD27 antibody CL647-65132Download protocol
Standard Protocols
Click here to view our Standard Protocols