Anticorps Monoclonal anti-PD-L1/CD274

PD-L1/CD274 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris et plus (1)

Applications

IHC, IF, FC

Conjugaison

APC Fluorescent Dye

Publications(4)

CloneNo.

10F.9G2

N° de cat : APC-65073

Synonymes

Cd274, PD-L1, 10F.9G2, B7 H1, PD L1

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Galerie de données de validation

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  FC experiment of mouse splenocytes using APC-65073

  1X10^6 unstimulated or anti-CD3/CD28 stimulated (2 days) BALB/C mouse splenocytes were surface stained with 0.1 ug APC Anti-Mouse CD274 (PD-L1, B7-H1) (10F.9G2) (APC-65073, Clone:10F.9G2). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: Anti-CD3/CD28 treated mouse splenocytes

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below
• FC: See 2 publications below

Informations sur le produit

APC-65073 cible PD-L1/CD274 dans les applications de IHC, IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: CD274 antigen
• Numéro d’acquisition GenBank: BC066841
• Symbole du gène: PD-L1
• Identification du gène (NCBI): 60533
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.

Publications

Species	Application	Title
	FC	Cell Signal; Cancer-associated fibroblasts-induced remodeling of tumor immune microenvironment via Jagged1 in glioma; Authors - Qing Zhang
mouse	FC	Adv Mater; Elucidation of Spatial Cooperativity in Chemo-Immunotherapy by a Sequential Dual-pH-Responsive Drug Delivery System; Authors - Shihao Wang
human	IHC,IF	Cell Metab; Itaconate transporter SLC13A3 confers immunotherapy resistance via alkylation-mediated stabilization of PD-L1; Authors - Yizeng Fan
		Adv Mater; An OMV-Based Nanovaccine as Antigen Presentation Signal Enhancer for Cancer Immunotherapy; Authors - Yichao Lu

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