Anticorps Monoclonal anti-CD3e

CD3e Monoclonal Antibody for FC

Hôte / Isotype

Hamster / IgG

Réactivité testée

souris

Applications

FC

Conjugaison

APC Fluorescent Dye

Publications(4)

CloneNo.

145-2C11

N° de cat : APC-65060

Synonymes

145-2C11, AI504783, CD3, CD3epsilon, CD3ε

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Galerie de données de validation

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  FC experiment of mouse splenocytes using APC-65060

  1x10^6 mouse splenocytes were surface stained with 0.2 ug APC Anti-Mouse CD3ε (145-2C11) (APC-65060, Clone:145-2C11) or 0.2 ug Isotype Control. Cells were co-stained with PE Anti-Mouse CD4 (GK1.5) (PE-65104, Clone: GK1.5). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 4 publications below

Informations sur le produit

APC-65060 cible CD3e dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Hamster / IgG
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Clone de lymphocyte T cytotoxique (CTL) murin spécifique de H-2Kb, BM10-37
• Nom complet: CD3 antigen, epsilon polypeptide
• Numéro d’acquisition GenBank: BC098236
• Symbole du gène: CD3ε
• Identification du gène (NCBI): 12501
• Conjugaison: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Publications

Species	Application	Title
mouse	FC	ACS Appl Mater Interfaces; Intelligent Nanoplatform Integrating Macrophage and Cancer Cell Membrane for Synergistic Chemodynamic/Immunotherapy/Photothermal Therapy of Breast Cancer; Authors - Shichao Zhang
	FC	Eur J Med Res; Dickkopf-related protein 3 alters aerobic glycolysis in pancreatic cancer BxPC-3 cells, promoting CD4+ T-cell activation and function.; Authors - Qingqu Guo
mouse	FC	J Adv Res; Design of a targeted dual drug delivery system for boosting the efficacy of photoimmunotherapy against melanoma proliferation and metastasis; Authors - Yi Chen
	FC	J Pharm Biomed Anal; Altered gut metabolites and metabolic reprogramming involved in the pathogenesis of colitis-associated colorectal cancer and the transition of colon "inflammation to cancer"; Authors - Dunfang Wang

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