Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

17A2

N° de cat : CL555-65077

Synonymes

Cd3e, 17A2, AI504783, CD3epsilon, CD3ε

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Galerie de données de validation

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  FC experiment of mouse splenocytes using CL555-65077

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4, and 0.25 ug CoraLite®555 Anti-Mouse CD3 (17A2) (CL555-65077, Clone:17A2) or CoraLite® Plus 555 Rat IgG2b Isotype Control (LTF-2) (CL555-65211, Clone: LTF-2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using CL555-65077

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite®555 Anti-Mouse CD3 (17A2) (CL555-65077, Clone:17A2) (red) or CoraLite® Plus 555 Rat IgG2b Isotype Control (LTF-2) (CL555-65211, Clone: LTF-2) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-65077 cible CD3 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Hybridome T-T γδTCR-positif D1
• Nom complet: CD3 antigen, epsilon polypeptide
• Numéro d’acquisition GenBank: BC098236
• Symbole du gène: CD3ε
• Identification du gène (NCBI): 12501
• Conjugaison: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

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