Anticorps Monoclonal anti-CD3

CD3 Monoclonal Antibody for FC, IF

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

IF, FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

17A2

N° de cat : CL647-65077

Synonymes

AI504783, CD3, Cd3e, CD3epsilon, T3e

---

Galerie de données de validation

• 

  IF Staining of mouse splenocytes using CL647-65077

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2) at dilution of 1:500.

• 

  FC experiment of C57BL/6 mouse splenocytes using CL647-65077

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with PE Anti-Mouse CD4 (GK1.5) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone:17A2) or 0.5 ug CoraLite® Plus 647 Rat IgG2b Isotype Control (LTF-2) (CL647-65211, Clone: LTF-2). Cells were not fixed.

---

Applications testées

• Résultats positifs en IF: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF): IF : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65077 cible CD3 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Hybridome T-T γδTCR-positif D1
• Nom complet: CD3 antigen, epsilon polypeptide
• Numéro d’acquisition GenBank: BC098236
• Symbole du gène: Cd3e
• Identification du gène (NCBI): 12501
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS avec azoture de sodium à 0,1 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).