Anticorps Monoclonal anti-CD31

CD31 Monoclonal Antibody for FC, IF

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris et plus (1)

Applications

IF, FC

Conjugaison

FITC Plus Fluorescent Dye

Publications

4

CloneNo.

390

N° de cat : FITC-65058

Synonymes

C85791, Cd31, Pecam, PECAM 1, Pecam1

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Galerie de données de validation

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  IF Staining of bEnd.3 using FITC-65058

  Immunofluorescent analysis of (4% PFA) fixed bEnd.3 cells using FITC Anti-Mouse CD31 (FITC-65058, Clone: 390) at dilution of 1:100.

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  FC experiment of C57BL/6 mouse bone marrow cells using FITC-65058

  1X10^6 C57BL/6 mouse bone marrow cells were surface co-stained with APC Anti-Mouse CD45R (B220) and 0.5 ug FITC Plus Anti-Mouse CD31 (FITC-65058, Clone: 390) or 0.5 ug FITC Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3). Cells were not fixed.

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Applications testées

• Résultats positifs en IF: cellules bEnd.3,
• Résultats positifs en cytométrie: cellules médullaires de souris

Dilution recommandée

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below
• FC: See 3 publications below

Informations sur le produit

FITC-65058 cible CD31 dans les applications de IF, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Lignée de cellules progénitrices hématopoïétiques 32D de souris C3H/HeJ
• Nom complet: platelet/endothelial cell adhesion molecule 1
• Numéro d’acquisition GenBank: BC008519
• Symbole du gène: Pecam1
• Identification du gène (NCBI): 18613
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
mouse	IF	Cell Death Dis; LncRNA ANRIL mediates endothelial dysfunction through BDNF downregulation in chronic kidney disease.; Authors - Hong Su
mouse	FC	Bone; mTORC1 coordinates NF-κB signaling pathway to promote chondrogenic differentiation of tendon cells in heterotopic ossification; Authors - Jiaming Fu
rat	FC	Cells; Enhancement of Tendon Repair Using Tendon-Derived Stem Cells in Small Intestinal Submucosa via M2 Macrophage Polarization; Authors - Xufeng Mao
mouse	FC	Biochim Biophys Acta Mol Cell Res; CARM1 deficiency inhibits osteoblastic differentiation of bone marrow mesenchymal stem cells and delays osteogenesis in mice; Authors - Jing-Yi Li