Anticorps Monoclonal anti-CD35

CD35 Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG1, kappa

Réactivité testée

Humain

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

CloneNo.

E11

N° de cat : FITC-65254

Synonymes

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN



Applications testées

Résultats positifs en cytométrieCMSP humaines,

Dilution recommandée

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Informations sur le produit

FITC-65254 cible CD35 dans les applications de FC et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1, kappa
Clonalité Monoclonal
Type Anticorps
Immunogène Human CD35 from Acute monocytic leukemia cells and normal blood monocytes
Nom complet complement component (3b/4b) receptor 1 (Knops blood group)
Masse moléculaire calculée 224 kDa
Numéro d’acquisition GenBankNM_000573
Symbole du gène CR1
Identification du gène (NCBI) 1378
Conjugaison FITC Plus Fluorescent Dye
Excitation/Emission maxima wavelengths495 nm / 524 nm
Forme Liquide
Méthode de purification Purification par affinité
Tampon de stockage PBS with 0.09% sodium azide and 0.5% BSA.
Conditions de stockageStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes.

Protocole

Product Specific Protocols
FC protocol for FITC Plus CD35 antibody FITC-65254Download protocol
Standard Protocols
Click here to view our Standard Protocols