Anticorps Monoclonal anti-CD4

CD4 Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

IF/ICC, FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

Publications(2)

CloneNo.

GK1.5

N° de cat : CL647-65104

Synonymes

Ly 4, L3T4, GK1.5, CD4 antigen

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Galerie de données de validation

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  IF Staining of mouse splenocytes using CL647-65104

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) at dilution of 1:500.

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  FC experiment of mouse splenocytes using CL647-65104

  1X10^6 mouse splenocytes were surface stained with FITC Anti-Mouse CD3 (FITC-65077, Clone: 17A2) and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD4 (CL647-65104, Clone: GK1.5) or CoraLite® Plus 647-Conjugated rat IgG2b isotype control. Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 2 publications below

Informations sur le produit

CL647-65104 cible CD4 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Clone V4 de CTL de souris
• Nom complet: CD4 antigen
• Numéro d’acquisition GenBank: BC039137
• Symbole du gène: Cd4
• Identification du gène (NCBI): 12504
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publications

Species	Application	Title
	IF	Front Chem; Activated platelet membrane nanovesicles recruit neutrophils to exert the antitumor efficiency; Authors - Yinghui Shang
mouse	IF	J Transl Med; HMGB1/TREM2 positive feedback loop drives the development of radioresistance and immune escape of glioblastoma by regulating TLR4/Akt signaling; Authors - Hui Qiu

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