Anticorps Monoclonal anti-CD4

CD4 Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

FITC Plus Fluorescent Dye

Publications(3)

CloneNo.

RM4-5

N° de cat : FITC-65141

Synonymes

CD4 antigen, L3T4, Ly 4, RM4-5, T-cell differentiation antigen L3T4

---

Galerie de données de validation

• 

  FC experiment of mouse splenocytes using FITC-65141

  1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3, and 0.25 ug FITC Plus Anti-Mouse CD4 (RM4-5) (FITC-65141, Clone: RM4-5) or FITC Plus Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-65141

  1x10^6 mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD4 (RM4-5) (FITC-65141, Clone: RM4-5) (red) or FITC Plus Rat IgG2a Isotype Control (2A3) (FITC-65209, Clone: 2A3) (blue). Cells were not fixed.

---

Applications testées

• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Applications publiées

• FC: See 3 publications below

Informations sur le produit

FITC-65141 cible CD4 dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: thymocytes de souris BALB/c
• Nom complet: CD4 antigen
• Numéro d’acquisition GenBank: BC039137
• Symbole du gène: Cd4
• Identification du gène (NCBI): 12504
• Conjugaison: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publications

Species	Application	Title
mouse	FC	Adv Healthc Mater; Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization; Authors - Hongping Wan
mouse	FC	Int J Biol Macromol; Mucoadhesive chitosan-catechol as an efficient vaccine delivery system for intranasal immunization; Authors - Kai Deng
mouse	FC	J Ethnopharmacol; Ethyl acetate extract from Herpetospermun cardigerum Wall. Ameliorated Concanavalin A-induced autoimmune hepatitis in mice by reprofiling gut microenvironment to modulate IDO1/KYN and PI3K/AKT/NF-κB pathways; Authors - Yu Xiao

{{ptg:RelatedPrimaryAntibodies}}