Anticorps Monoclonal anti-CD45

CD45 Monoclonal Antibody for IF/ICC, FC

Hôte / Isotype

Rat / IgG2b, kappa

Réactivité testée

souris

Applications

IF/ICC, FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

Publications(1)

CloneNo.

30-F11

N° de cat : CL647-65087

Synonymes

Ptprc, 30-F11, EC:3.1.3.48, LCA, L-CA

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Galerie de données de validation

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  IF Staining of mouse splenocytes using CL647-65087

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse CD45 (CL647-65087, Clone: 30-F11) at dilution of 1:400.

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  FC experiment of mouse splenocytes using CL647-65087

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD45 (CL647-65087, Clone: 30-F11) (red) or isotype control antibody (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en IF/ICC: splénocytes de souris,
• Résultats positifs en cytométrie: splénocytes de souris,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

CL647-65087 cible CD45 dans les applications de IF/ICC, FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Thymus ou rate de souris
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC028512
• Symbole du gène: CD45
• Identification du gène (NCBI): 19264
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publications

Species	Application	Title
	IF	Cell Rep; Inflammatory cytokines mediate the induction of and awakening from metastatic dormancy; Authors - Paulo Pereira

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