Anticorps Monoclonal anti-CD45RA

CD45RA Monoclonal Antibody for IF-P, FC

Hôte / Isotype

Mouse / IgG2b, kappa

Réactivité testée

Humain

Applications

IF-P, FC

Conjugaison

PE Fluorescent Dye

CloneNo.

HI100

N° de cat : PE-65108

Synonymes

CD45, EC:3.1.3.48, GP180, HI100, L CA

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Galerie de données de validation

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  IF Staining of human tonsillitis using PE-65108

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using PE CD45RA antibody (PE-65108, Clone: HI100 ) at dilution of 1:10. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of human PBMCs using PE-65108

  1X10^6 human PBMCs were surface co-stained with CL647 Anti-Human CD45RO and 5 ul PE Anti-Human CD45RA (PE-65108, Clone:HI100) or PE Mouse IgG2b Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

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  FC experiment of human PBMCs using PE-65108

  1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD45RA (PE-65108, Clone:HI100) or PE Mouse IgG2b Isotype Control. Cells were not fixed. CD3+ lymphocytes were gated.

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Applications testées

• Résultats positifs en IF-P: tissu d'amygdalite humain,
• Résultats positifs en cytométrie: CMSP humaines,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:10-1:100

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Informations sur le produit

PE-65108 cible CD45RA dans les applications de IF-P, FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: s/o
• Nom complet: protein tyrosine phosphatase, receptor type, C
• Numéro d’acquisition GenBank: BC014239
• Symbole du gène: CD45
• Identification du gène (NCBI): 5788
• Conjugaison: PE Fluorescent Dye
• Excitation/Emission maxima wavelengths: 496 nm, 565 nm / 578 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

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