Anticorps Monoclonal anti-CHOP; GADD153

CHOP; GADD153 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

CloneNo.

4D5A9

N° de cat : 60304-1-Ig

Synonymes

CEBPZ, CHOP, CHOP 10, CHOP10, DDIT 3, DDIT3, GADD153

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Galerie de données de validation

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  WB analysis of HeLa using 60304-1-Ig

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 60304-1-Ig (CHOP; GADD153 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la tunicamycine

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

60304-1-Ig cible CHOP; GADD153 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CHOP; GADD153 Protéine recombinante Ag7354
• Nom complet: DNA-damage-inducible transcript 3
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC003637
• Symbole du gène: DDIT3
• Identification du gène (NCBI): 1649
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).

Publications

Species	Application	Title
human	WB	Autophagy; Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.; Authors - Tengfei Huang
human	WB,IHC	Cell Death Dis; YAP1 enhances NF-κB-dependent and independent effects on clock-mediated unfolded protein responses and autophagy in sarcoma.; Authors - Adrian Rivera-Reyes
rat	WB	Hum Mol Genet; The role of the ER stress-response protein PERK in rhodopsin retinitis pigmentosa.; Authors - Dimitra Athanasiou
human, mouse	WB	Oncotarget; Deptor transcriptionally regulates endoplasmic reticulum homeostasis in multiple myeloma cells.; Authors - Valeria Catena
human	WB	Apoptosis; Honokiol induces paraptosis-like cell death of acute promyelocytic leukemia via mTOR & MAPK signaling pathways activation.; Authors - Xiaoli Liu
human	WB	J Virol; ATF6-mediated unfolded protein response facilitates AAV2 transduction by releasing the suppression of AAV receptor on ER stress; Authors - Mengtian Cui