Anticorps Polyclonal de lapin anti-CXCR1

• Phare
• Validé par KD/KO

CXCR1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 55450-1-AP

Synonymes

IL8RA, C C, C C CKR 1, CD128, CD181

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Galerie de données de validation

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  WB analysis of Jurkat using 55450-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55450-1-AP (CXCR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 55450-1-AP

  IP result of anti-CXCR1 (IP:55450-1-AP, 4ug; Detection:55450-1-AP 1:500) with Jurkat cells lysate 4000ug.

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  IF Staining of Jurkat using 55450-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR1 antibody (55450-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules Jurkat,
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 1 publications below

Informations sur le produit

55450-1-AP cible CXCR1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: interleukin 8 receptor, alpha
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: NM_000634
• Symbole du gène: CXCR1
• Identification du gène (NCBI): 3577
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CXCR1, also named CMKAR1, IL8RA, and CD181, belongs to the G-protein coupled receptor 1 family. It is a receptor to interleukin-8, a powerful neutrophil chemotactic factor. The binding of IL-8 to the receptor causes activation of neutrophils. CXCR1 binds to IL-8 with a high affinity and to MGSA (GRO) with a low affinity. This antibody is specific to CXCR1.

Publications

Species	Application	Title
mouse	WB	Cell Death Dis; CXCR1 drives the pathogenesis of EAE and ARDS via boosting dendritic cells-dependent inflammation; Authors - Wei Zhuang
mouse	WB,IF	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin ; KD Validated
human	WB	Lung Cancer; Combination therapy with anti-programmed cell death 1 antibody plus angiokinase inhibitor exerts synergistic antitumor effect against malignant mesothelioma via tumor microenvironment modulation; Authors - Akio Tada
mouse	WB	Cytokine; IL-8 exacerbates alcohol-induced fatty liver disease via the Akt/HIF-1α pathway in human IL-8-expressing mice.; Authors - Zhihao Wang
mouse	WB	J Agric Food Chem; Oral Intervention of Narirutin Ameliorates the Allergic Response of Ovalbumin Allergy; Authors - Xiaolei Shi
mouse	WB	Mol Immunol; IL-8 exacerbates CCl4-induced liver fibrosis in human IL-8-expressing mice via the PI3K/Akt/HIF-1α pathway; Authors - Yuchen Ma

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