Anticorps Polyclonal de lapin anti-DDX10

• Phare
• Validé par KD/KO

DDX10 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 17857-1-AP

Synonymes

DDX10, DEAD box protein 10, HRH J8

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Galerie de données de validation

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  WB analysis of SMMC-7721 using 17857-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17857-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse testis using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17857-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules SMMC-7721, cellules HeLa
• Résultats positifs en IHC: tissu testiculaire de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

17857-1-AP cible DDX10 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DDX10 Protéine recombinante Ag12317
• Nom complet: DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
• Masse moléculaire calculée: 875 aa, 101 kDa
• Poids moléculaire observé: 101 kDa
• Numéro d’acquisition GenBank: BC091521
• Symbole du gène: DDX10
• Identification du gène (NCBI): 1662
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440).

Publications

Species	Application	Title
human	WB,IHC	Cancer Cell Int; DDX10 promotes the proliferation and metastasis of colorectal cancer cells via splicing RPL35.; Authors - Xin Zhou ; KD Validated
human	WB	Biochem Biophys Res Commun; Epigenetic down-regulated DDX10 promotes cell proliferation through Akt/NF-κB pathway in ovarian cancer.; Authors - Muhuizi Gai ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wael (Verified Customer) (05-24-2023)

We detected the DDX10 relative abundance in both the nuclear and cytoplasmic fractions

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293

DDX10 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:17857-1-AP)