Anticorps Polyclonal de lapin anti-E2F3

E2F3 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12334-1-AP

Synonymes

E2F3, Q96AR0

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Galerie de données de validation

• 

  WB analysis of Y79 using 12334-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12334-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12334-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12334-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse liver using 12334-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12334-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12334-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12334-1-AP (E2F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12334-1-AP

  IP Result of anti-E2F3 (IP:12334-1-AP, 4ug; Detection:12334-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules Y79, cellules HEK-293, cellules HepG2, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

12334-1-AP cible E2F3 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: E2F3 Protéine recombinante Ag3039
• Nom complet: E2F transcription factor 3
• Masse moléculaire calculée: 133 aa, 15 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC016847
• Symbole du gène: E2F3
• Identification du gène (NCBI): 1871
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

E2F transcription factors regulate gene expression in concert with the retinoblastoma tumor suppressor family. These transcriptional complexes are master regulators of cell cycle progression, control the expression of genes involved in DNA repair, G2/M checkpoint and differentiation [PMID:22580460]. E2f3 is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F3 binds specifically to RB1 in a cell-cycle dependent manner [PMID:22960857].

Publications

Species	Application	Title
human	WB, IHC	Nat Commun; POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation.; Authors - Boshi Wang
human	WB	Mol Med Rep; [Corrigendum] Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells.; Authors - Dacai Xu
human	WB	Mol Med Rep; Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells.; Authors - Dacai Xu