Anticorps Polyclonal de lapin anti-EGR1

• Phare
• Validé par KD/KO

EGR1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

25

N° de cat : 22008-1-AP

Synonymes

AT225, early growth response 1, EGR 1, EGR1, G0S30, KROX 24, KROX24, NGFI A, TIS8, Transcription factor ETR103, Transcription factor Zif268, ZIF 268, Zinc finger protein 225, Zinc finger protein Krox 24, ZNF225

---

Galerie de données de validation

• 

  WB analysis using 22008-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22008-1-AP (EGR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22008-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22008-1-AP (EGR1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 22008-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22008-1-AP (EGR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 22008-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22008-1-AP (EGR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 22008-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 22008-1-AP (EGR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 22008-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 22008-1-AP (EGR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules Ramos, RAW264.7
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 20 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• ELISA: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

22008-1-AP cible EGR1 dans les applications de WB, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EGR1 Protéine recombinante Ag13395
• Nom complet: early growth response 1
• Masse moléculaire calculée: 543 aa, 58 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC073983
• Symbole du gène: EGR1
• Identification du gène (NCBI): 1958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EGR1, also named as Early growth response protein 1, is a 543 amino acid protein, which belongs to the EGR C2H2-type zinc-finger protein family. EGR1 as a transcriptional regulator recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes and binds double-stranded target DNA, irrespective of the cytosine methylation status. EGR1 regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. EGR1 activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation and plays a role in the regulation of cell survival, proliferation and cell death. Egr1 is suggested to be a 55-kDa protein according to the translation of its coding sequence. Analysis of cytoplasmic and nuclear fractions of normal and irradiated native CNS tissue by Western blotting revealed a 110-kDa band for Egr1 localized in the cytoplasm and a 75-kDa version for the nuclear Egr1(PMID: 17497096).

Publications

Species	Application	Title
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv ; KD Validated
mouse	WB	Mol Ther Nucleic Acids; Genetic or siRNA inhibition of MBD2 attenuates the UUO- and I/R-induced renal fibrosis via downregulation of EGR1.; Authors - Kai Ai ; KD Validated
human	WB	Pharmacol Res; Discovery of a novel megakaryopoiesis enhancer, ingenol, promoting thrombopoiesis through PI3K-Akt signaling independent of thrombopoietin.; Authors - Long Wang
human	WB	J Cell Mol Med; EGR1 promoted anticancer effects of Scutellarin via regulating LINC00857/miR-150-5p/c-Myc in osteosarcoma.; Authors - Jian Han ; KD Validated
rat	IF	Phytomedicine; Stigmasterol upregulates immediate early genes and promotes neuronal cytoarchitecture in primary hippocampal neurons as revealed by transcriptome analysis.; Authors - Md Nazmul Haque
mouse	WB,IHC	Front Pharmacol; Tilianin Reduces Apoptosis via the ERK/EGR1/BCL2L1 Pathway in Ischemia/Reperfusion-Induced Acute Kidney Injury Mice.; Authors - Zengying Liu