Anticorps Polyclonal de lapin anti-ENT1

• Phare
• Validé par KD/KO

ENT1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

31

Avis (1)

N° de cat : 11337-1-AP

Synonymes

ENT1, SLC29A1

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Galerie de données de validation

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  WB analysis of mouse brain using 11337-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11337-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11337-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11337-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11337-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11337-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11337-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11337-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11337-1-AP (ENT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11337-1-AP

  IP Result of anti-ENT1 (IP:11337-1-AP, 3ug; Detection:11337-1-AP 1:400) with mouse brain tissue lysate 8000ug.

• 

  IHC staining of human breast cancer using 11337-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11337-1-AP (ENT1 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu hépatique de souris, tissu hépatique humain, tissu pulmonaire de souris, tissu pulmonaire humain, tissu rénal de souris, tissu splénique humain, tissu testiculaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 21 publications below
• IHC: See 13 publications below
• IF: See 1 publications below

Informations sur le produit

11337-1-AP cible ENT1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ENT1 Protéine recombinante Ag1881
• Nom complet: solute carrier family 29 (nucleoside transporters), member 1
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC008954
• Symbole du gène: SLC29A1
• Identification du gène (NCBI): 2030
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ENT1 (equilibrative nucleoside transporter 1; also known as SLC29A1) is a ubiquitous protein located at the cell membrane and is a major transporter responsible for the cellular uptake and release of endogenous nucleosides such as adenosine, and nucleoside analogs used in chemotherapy. The predicted molecular weight of ENT1 is 50 kDa, while heavier 54-62 kDa band may be observed due to the glycosylation (16448802, 11850433, 16111480). A smaller novel splice variant of the mouse ENT1 has also been identified, which generates a protein of 35-40 kDa (18413666).

Publications

Species	Application	Title
human	WB	Cell; Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.; Authors - Kazuhiro Koikawa ; KD Validated
human	WB	Gastroenterology; ZIP4 Increases Expression of Transcription Factor ZEB1 to Promote Integrin α3β1 Signaling and Inhibit Expression of the Gemcitabine Transporter ENT1 in Pancreatic Cancer Cells.; Authors - Mingyang Liu
human	WB,IHC	Theranostics; The failure of DAC to induce OCT2 expression and its remission by hemoglobin-based nanocarriers under hypoxia in renal cell carcinoma.; Authors - Lu Chen ; KD Validated
human	WB,IHC	Oncogene; Abrogation of glutathione peroxidase-1 drives EMT and chemoresistance in pancreatic cancer by activating ROS-mediated Akt/GSK3β/Snail signaling.; Authors - Qingcai Meng
mouse	WB	Br J Pharmacol; PMID: 31166004; Authors - Zhiyuan Ma
human	WB	Am J Cancer Res; PMID: 32368394; Authors - Zeng Ye

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Parima (Verified Customer) (05-09-2023)

Since we received it in January, I have been trying to troubleshoot our western as there were so many non-specific bands so it was hard to tell which bands exactly are ENT1 bands. I have probed for ENT1 using this antibody in pancreatic cancer cell lines (BxPC3, MIA PaCa-2 and Panc-1) as well as in MDA-MB-231 breast cancer cell lines and the results were not good. I have been doing western for several years and this is the first antibody that I have a hard time troubleshooting to get nice clean bands and I even probed for ENT1 first. Can you please check if this specific lot has a problem? We had to wait for this product for so long because it was backordered and it's very disappointed to get the results we got.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 - 1:2500
• Cell Tissue Type: BxPC3, PANC-1, MIA PaCa-2, MDA-MB-231

ENT1 Antibody Western Blot validation (1:1000 - 1:2500 dilution) in BxPC3, PANC-1, MIA PaCa-2, MDA-MB-231 (Cat no:11337-1-AP)

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