Anticorps Monoclonal anti-Fibromodulin

• Phare
• Validé par KD/KO

Fibromodulin Monoclonal Antibody for IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(13)

CloneNo.

3E9D10

N° de cat : 60108-1-Ig

Synonymes

fibromodulin, FM, FMOD, KSPG fibromodulin, SLRR2E

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Galerie de données de validation

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  WB analysis of human kidney using 60108-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60108-1-Ig (Fibromodulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 60108-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 60108-1-Ig (Fibromodulin antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

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  WB analysis of human stomach using 60108-1-Ig

  human stomach tissue were subjected to SDS PAGE followed by western blot with 60108-1-Ig (Fibromodulin antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

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  IHC staining of human hepatocirrhosis using 60108-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 60108-1-Ig (Fibromodulin antibody) at dilution of 1:300 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 60108-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 60108-1-Ig (Fibromodulin antibody) at dilution of 1:300 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu de cirrhose hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 5 publications below
• IF: See 4 publications below

Informations sur le produit

60108-1-Ig cible Fibromodulin dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Fibromodulin Protéine recombinante Ag4030
• Nom complet: fibromodulin
• Masse moléculaire calculée: 376 aa, 43 kDa
• Numéro d’acquisition GenBank: BC035281
• Symbole du gène: Fibromodulin
• Identification du gène (NCBI): 2331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Fibromodulin (FMOD), an extracellular matrix protein, belongs to the small leucine-rich proteoglycan family. Fibromodulin is a collagen-binding protein present in many types of connective tissues, e.g. cartilage, tendon, skin, sclera and cornea (PMID: 2531085). It is involved in fibrillogenesis, cell adhesion, and cytokine activity modulation (PMID: 15741214). Fibromodulin may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. The calculated molecular mass of fibromodulin is 43 kDa, extracellular fibromodulin appears as one single band of 59 kDa due to glycosylation (PMID: 2531085; 22138190).

Publications

Species	Application	Title
mouse	IHC	Adv Sci (Weinh); Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution; Authors - Taifeng Zhou
mouse	IHC	J Clin Invest; Melanocyte-secreted fibromodulin promotes an angiogenic microenvironment.; Authors - Irit Adini ; KD Validated
mouse	IF	Acta Biomater; In situ regeneration of bone-to-tendon structures: Comparisons between costal-cartilage derived stem cells and BMSCs in the rat model.; Authors - Rui Zuo
human		FASEB J; Melanocyte pigmentation inversely correlates with MCP-1 production and angiogenesis-inducing potential.; Authors - Irit Adini
human,mouse	WB,IF	BMC Cancer; Multi-omics sequencing revealed endostar combined with cisplatin treated non small cell lung cancer via anti-angiogenesis; Authors - Yufei Wang
rat	WB	Cartilage; Enhancement and Repair of Degenerative Intervertebral Disc in Rats Using Platelet-Rich Plasma/Ferulic Acid Hydrogel; Authors - Qiang Chai

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