Anticorps Polyclonal de lapin anti-GBX2

• Phare
• Validé par KD/KO

GBX2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, chIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 21639-1-AP

Synonymes

Homeobox protein GBX-2, Gastrulation and brain-specific homeobox protein 2

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Galerie de données de validation

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  WB analysis of HeLa using 21639-1-AP

  WB result of GBX2 antibody (21639-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected HeLa cells.

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  WB analysis of mouse stomach using 21639-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21639-1-AP (GBX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21639-1-AP

  IP result of anti-GBX2 (IP:21639-1-AP, 4ug; Detection:21639-1-AP 1:300) with HeLa cells lysate 3000ug.

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  IF Staining of HeLa using 21639-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GBX2 antibody (21639-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu d'estomac de souris, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• ChIP: See 2 publications below

Informations sur le produit

21639-1-AP cible GBX2 dans les applications de WB, IHC, IF/ICC, IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GBX2 Protéine recombinante Ag16288
• Nom complet: gastrulation brain homeobox 2
• Masse moléculaire calculée: 348 aa, 37 kDa
• Poids moléculaire observé: 33-37 kDa
• Numéro d’acquisition GenBank: BC137448
• Symbole du gène: GBX2
• Identification du gène (NCBI): 2637
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Homeobox protein GBX-2 (GBX-2) is a homeobox gene essential for normal development of the midbrain and the anterior hindbrain. It mainly expressed in the brain, and located in nucleoplasm. It is presumably a transcription factor that is likely to regulate the expression of genes involved in establishing early A/P pattern in the neural plate (PMID: 9247335).

Publications

Species	Application	Title
human	IF	Cell Stem Cell; Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders; Authors - David Shin
human	chIP	RNA Biol; NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.; Authors - Xiwen Li
human	WB,ChIP	Funct Integr Genomics; Oncogenic GBX2 promotes the malignant behaviors of bladder cancer cells by binding to the ITGA5 promoter and activating its transcription.; Authors - Yaoyao Xiong
human	IF	Life Med; CRISPR/Cas9-mediated genetic correction reverses spinocerebellar ataxia 3 disease-associated phenotypes in differentiated cerebellar neurons; Authors - Guoxu Song
human	IHC,IF	Front Neuroanat; Development of the early fetal human thalamus: from a protomap to emergent thalamic nuclei; Authors - Maznah Alhesain

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