Anticorps Polyclonal de lapin anti-Connexin-43

Connexin-43 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

34

N° de cat : 15386-1-AP

Synonymes

Connexin-43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD

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Galerie de données de validation

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  WB analysis of HeLa using 15386-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15386-1-AP (GJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15386-1-AP

  IP Result of anti-Connexin-43 (IP:15386-1-AP, 4ug; Detection:15386-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of mouse heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 15386-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15386-1-AP (GJA1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15386-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 15386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 15386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 15386-1-AP (Connexin-43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 15386-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug Connexin-43 antibody (15386-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, tissu cardiaque de souris
• Résultats positifs en cytométrie: cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 18 publications below
• IHC: See 4 publications below
• IF: See 17 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

15386-1-AP cible Connexin-43 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Connexin-43 Protéine recombinante Ag6698
• Nom complet: gap junction protein, alpha 1, 43kDa
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43-47 kDa
• Numéro d’acquisition GenBank: BC026329
• Symbole du gène: GJA1
• Identification du gène (NCBI): 2697
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Connexin-43 (Cx43, also known as gap junction alpha-1), a member of connexin family, plays essential roles in gap junction communication that facilitates direct communication among adjacent cells (PMID: 12270943). Usually, six connexin proteins oligomerize into a hemi-channel or connexon during intercellular channel formation (PMID: 12270943). Mutations of Cx43 may cause a series of diseases such as oculodentodigital dysplasia (ODDD), syndactyly 3 (SDTY3), hypoplastic left heart syndrome 1 (HLHS1) and so on (PMID: 18161618, 1472936, 1147490).

Publications

Species	Application	Title
human	IF	Nat Commun; Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation.; Authors - Yin Wang
mouse	IF	Thromb Res; Selective increase of cardiomyocyte derived extracellular vesicles after experimental myocardial infarction and functional effects on the endothelium.; Authors - Jose A Rodriguez
mouse	WB	Hypertension; Novel Role for the Immunoproteasome Subunit PSMB10 in Angiotensin II-Induced Atrial Fibrillation in Mice.; Authors - Jing Li
	IF	Ecotoxicol Environ Saf; Sodium Selenite inhibits mitophagy, downregulation and mislocalization of blood-testis barrier proteins of bovine Sertoli cell exposed to microcystin-leucine arginine (MC-LR) via TLR4/NF-kB and mitochondrial signaling pathways blockage.; Authors - E O Adegoke
human	WB	Environ Toxicol Pharmacol; Microcystin-leucine arginine (MC-LR) induced inflammatory response in bovine sertoli cell via TLR4/NF-kB signaling pathway.; Authors - E O Adegoke
human	IF	Free Radic Biol Med; Oxidative stress contributes to hepatocyte growth factor-dependent pro-senescence activity of ovarian cancer cells.; Authors - Justyna Mikuła-Pietrasik

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